Deuterium-enriched lenalidomide

ABSTRACT

The present application describes deuterium-enriched lenalidomide, pharmaceutically acceptable salt forms thereof, and methods of treating using the same.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. patent application Ser. No.13/607,785, filed Sep. 9, 2012; which is a continuation of U.S. patentapplication Ser. No. 12/196,672, filed Aug. 22, 2008, now U.S. Pat. No.8,288,414, issued Oct. 16, 2012; which claims priority benefit under 35U.S.C. §119(e) of U.S. Provisional Patent Application Ser. No.60/971,891 filed Sep. 12, 2007. The disclosure of each of which isincorporated herein by reference in its entirety.

FIELD OF THE INVENTION

This invention relates generally to deuterium-enriched lenalidomide,pharmaceutical compositions containing the same, and methods of usingthe same.

BACKGROUND OF THE INVENTION

Lenalidomide, shown below, is a well known bortezomib.

Since lenalidomide is a known and useful pharmaceutical, it is desirableto discover novel derivatives thereof. Lenalidomide is described in U.S.Pat. No. 5,635,517; the contents of which are incorporated herein byreference.

SUMMARY OF THE INVENTION

Accordingly, one object of the present invention is to providedeuterium-enriched lenalidomide or a pharmaceutically acceptable saltthereof.

It is another object of the present invention to provide pharmaceuticalcompositions comprising a pharmaceutically acceptable carrier and atherapeutically effective amount of at least one of thedeuterium-enriched compounds of the present invention or apharmaceutically acceptable salt thereof.

It is another object of the present invention to provide a method fortreating multiple myeloma, comprising administering to a host in need ofsuch treatment a therapeutically effective amount of at least one of thedeuterium-enriched compounds of the present invention or apharmaceutically acceptable salt thereof

It is another object of the present invention to provide a noveldeuterium-enriched lenalidomide or a pharmaceutically acceptable saltthereof for use in therapy.

It is another object of the present invention to provide the use of anovel deuterium-enriched lenalidomide or a pharmaceutically acceptablesalt thereof for the manufacture of a medicament (e.g., for thetreatment of multiple myeloma).

These and other objects, which will become apparent during the followingdetailed description, have been achieved by the inventor's discovery ofthe presently claimed deuterium-enriched lenalidomide.

DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS

Deuterium (D or ²H) is a stable, non-radioactive isotope of hydrogen andhas an atomic weight of 2.0144. Hydrogen naturally occurs as a mixtureof the isotopes ¹H (hydrogen or protium), D (²H or deuterium), and T (³Hor tritium). The natural abundance of deuterium is 0.015%. One ofordinary skill in the art recognizes that in all chemical compounds witha H atom, the H atom actually represents a mixture of H and D, withabout 0.015% being D. Thus, compounds with a level of deuterium that hasbeen enriched to be greater than its natural abundance of 0.015%, shouldbe considered unnatural and, as a result, novel over their non-enrichedcounterparts.

All percentages given for the amount of deuterium present are molepercentages.

It can be quite difficult in the laboratory to achieve 100% deuterationat any one site of a lab scale amount of compound (e.g., milligram orgreater). When 100% deuteration is recited or a deuterium atom isspecifically shown in a structure, it is assumed that a small percentageof hydrogen may still be present. Deuterium-enriched can be achieved byeither exchanging protons with deuterium or by synthesizing the moleculewith enriched starting materials.

The present invention provides deuterium-enriched lenalidomide or apharmaceutically acceptable salt thereof. There are thirteen hydrogenatoms in the lenalidomide portion of lenalidomide as show by variablesR₁-R₁₃ in formula I below.

The hydrogens present on lenalidomide have different capacities forexchange with deuterium. Hydrogen atoms R₁-R₃ are easily exchangeableunder physiological conditions and, if replaced by deuterium atoms, itis expected that they will readily exchange for protons afteradministration to a patient. Hydrogen atoms R₉ and R₁₂-R₁₃ may beexchanged for deuterium atoms by the action of a suitable base such ast-BuOK/t-BuOD. The remaining hydrogen atoms are not easily exchangeablefor deuterium atoms. However, deuterium atoms at the remaining positionsmay be incorporated by the use of deuterated starting materials orintermediates during the construction of lenalidomide.

The present invention is based on increasing the amount of deuteriumpresent in lenalidomide above its natural abundance. This increasing iscalled enrichment or deuterium-enrichment. If not specifically noted,the percentage of enrichment refers to the percentage of deuteriumpresent in the compound, mixture of compounds, or composition. Examplesof the amount of enrichment include from about 0.5, 1, 2, 3, 4, 5, 6, 7,8, 9, 10, 12, 16, 21, 25, 29, 33, 37, 42, 46, 50, 54, 58, 63, 67, 71,75, 79, 84, 88, 92, 96, to about 100 mol %. Since there are 13 hydrogensin lenalidomide, replacement of a single hydrogen atom with deuteriumwould result in a molecule with about 8% deuterium enrichment. In orderto achieve enrichment less than about 8%, but above the naturalabundance, only partial deuteration of one site is required. Thus, lessthan about 8% enrichment would still refer to deuterium-enrichedlenalidomide.

With the natural abundance of deuterium being 0.015%, one would expectthat for approximately every 6,667 molecules of lenalidomide(1/0.00015=6,667), there is one naturally occurring molecule with onedeuterium present. Since lenalidomide has 13 positions, one wouldroughly expect that for approximately every 86,671 molecules oflenalidomide (13×6,667), all 13 different, naturally occurring,mono-deuterated lenalidomides would be present. This approximation is arough estimate as it doesn't take into account the different exchangerates of the hydrogen atoms on lenalidomide. For naturally occurringmolecules with more than one deuterium, the numbers become vastlylarger. In view of this natural abundance, the present invention, in anembodiment, relates to an amount of an deuterium enriched compound,whereby the enrichment recited will be more than naturally occurringdeuterated molecules.

In view of the natural abundance of deuterium-enriched lenalidomide, thepresent invention also relates to isolated or purifieddeuterium-enriched lenalidomide. The isolated or purifieddeuterium-enriched lenalidomide is a group of molecules whose deuteriumlevels are above the naturally occurring levels (e.g., 8%). The isolatedor purified deuterium-enriched lenalidomide can be obtained bytechniques known to those of skill in the art (e.g., see the synthesesdescribed below).

The present invention also relates to compositions comprisingdeuterium-enriched lenalidomide. The compositions require the presenceof deuterium-enriched lenalidomide which is greater than its naturalabundance. For example, the compositions of the present invention cancomprise (a) a μg of a deuterium-enriched lenalidomide; (b) a mg of adeuterium-enriched lenalidomide; and, (c) a gram of a deuterium-enrichedlenalidomide.

In an embodiment, the present invention provides an amount of a noveldeuterium-enriched lenalidomide.

Examples of amounts include, but are not limited to (a) at least 0.01,0.02, 0.03, 0.04, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, to 1 mole, (b) at least0.1 moles, and (c) at least 1 mole of the compound. The present amountsalso cover lab-scale (e.g., gram scale), kilo-lab scale (e.g., kilogramscale), and industrial or commercial scale (e.g., multi-kilogram orabove scale) quantities as these will be more useful in the actualmanufacture of a pharmaceutical. Industrial/commercial scale refers tothe amount of product that would be produced in a batch that wasdesigned for clinical testing, formulation, sale/distribution to thepublic, etc.

In another embodiment, the present invention provides a novel, deuteriumenriched compound of formula I or a pharmaceutically acceptable saltthereof.

wherein R₁-R₁₃ are independently selected from H and D; and theabundance of deuterium in R₁-R₁₃ is at least 8%. The abundance can alsobe (a) at least 15%, (b) at least 23%, (c) at least 31%,(d) at least38%, (e) at least 46%, (f) at least 54%, (g) at least 62%, (h) at least69%, (i) at least 77%, (j) at least 85%, (k) at least 92%, and (1) 100%.

In another embodiment, the present invention provides a novel, deuteriumenriched compound of formula I or a pharmaceutically acceptable saltthereof, wherein the abundance of deuterium in R₁-R₃ is at least 33%.The abundance can also be (a) at least 67%, and (b) 100%.

In another embodiment, the present invention provides a novel, deuteriumenriched compound of formula I or a pharmaceutically acceptable saltthereof, wherein the abundance of deuterium in R₉ and R₁₂-R₁₃ is atleast 33%. The abundance can also be (a) at least 67%, and (b) 100%.

In another embodiment, the present invention provides a novel, deuteriumenriched compound of formula I or a pharmaceutically acceptable saltthereof, wherein the abundance of deuterium in R₁-R₃, R₉, and R₁₂-R₁₃ isat least 17%. The abundance can also be (a) at least 33%, (b) at least50%, (c) at least 67%, (d) at least 83%, and (e) 100%.

In another embodiment, the present invention provides a novel, deuteriumenriched compound of formula I or a pharmaceutically acceptable saltthereof, wherein the abundance of deuterium in R₄-R₆ is at least 33%.The abundance can also be (a) at least 67%, and (b) 100%.

In another embodiment, the present invention provides a novel, deuteriumenriched compound of formula I or a pharmaceutically acceptable saltthereof, wherein the abundance of deuterium in R₇-R₈ is at least 50%.The abundance can also be (a) 100%.

In another embodiment, the present invention provides a novel, deuteriumenriched compound of formula I or a pharmaceutically acceptable saltthereof, wherein the abundance of deuterium in R₁₀-R₁₃ is at least 25%.The abundance can also be (a) at least 50%, (b) at least 75%, and (c)100%.

In another embodiment, the present invention provides an isolated novel,deuterium enriched compound of formula I or a pharmaceuticallyacceptable salt thereof.

wherein R₁-R₁₃ are independently selected from H and D; and theabundance of deuterium in R₁-R₁₃ is at least 8%. The abundance can alsobe (a) at least 15%, (b) at least 23%, (c) at least 31%,(d) at least38%, (e) at least 46%, (f) at least 54%, (g) at least 62%, (h) at least69%, (i) at least 77%, (j) at least 85%, (k) at least 92%, and (1) 100%.

In another embodiment, the present invention provides an isolated novel,deuterium enriched compound of formula I or a pharmaceuticallyacceptable salt thereof, wherein the abundance of deuterium in R₁-R₃ isat least 33%. The abundance can also be (a) at least 67%, and (b) 100%.

In another embodiment, the present invention provides an isolated novel,deuterium enriched compound of formula I or a pharmaceuticallyacceptable salt thereof, wherein the abundance of deuterium in R₉ andR₁₂-R₁₃ is at least 33%. The abundance can also be (a) at least 67%, and(b) 100%.

In another embodiment, the present invention provides an isolated novel,deuterium enriched compound of formula I or a pharmaceuticallyacceptable salt thereof, wherein the abundance of deuterium in R₁-R₃,R₉, and R₁₂-R₁₃ is at least 17%. The abundance can also be (a) at least33%, (b) at least 50%, (c) at least 67%, (d) at least 83%, and (e) 100%.

In another embodiment, the present invention provides an isolated novel,deuterium enriched compound of formula I or a pharmaceuticallyacceptable salt thereof, wherein the abundance of deuterium in R₄-R₆ isat least 33%. The abundance can also be (a) at least 67%, and (b) 100%.

In another embodiment, the present invention provides an isolated novel,deuterium enriched compound of formula I or a pharmaceuticallyacceptable salt thereof, wherein the abundance of deuterium in R₇-R₈ isat least 50%. The abundance can also be (a) 100%.

In another embodiment, the present invention provides an isolated novel,deuterium enriched compound of formula I or a pharmaceuticallyacceptable salt thereof, wherein the abundance of deuterium in R₁₀-R₁₃is at least 25%. The abundance can also be (a) at least 50%, (b) atleast 75%, and (c) 100%.

In another embodiment, the present invention provides novel mixture ofdeuterium enriched compounds of formula I or a pharmaceuticallyacceptable salt thereof.

wherein R₁-R₁₃ are independently selected from H and D; and theabundance of deuterium in R₁-R₁₃ is at least 8%. The abundance can alsobe (a) at least 15%, (b) at least 23%, (c) at least 31%,(d) at least38%, (e) at least 46%, (f) at least 54%, (g) at least 62%, (h) at least69%, (i) at least 77%, (j) at least 85%, (k) at least 92%, and (1) 100%.

In another embodiment, the present invention provides a novel mixtureof, deuterium enriched compound of formula I or a pharmaceuticallyacceptable salt thereof, wherein the abundance of deuterium in R₁-R₃ isat least 33%. The abundance can also be (a) at least 67%, and (b) 100%.

In another embodiment, the present invention provides a novel mixtureof, deuterium enriched compound of formula I or a pharmaceuticallyacceptable salt thereof, wherein the abundance of deuterium in R₉ andR₁₂-R₁₃ is at least 33%. The abundance can also be (a) at least 67%, and(b) 100%.

In another embodiment, the present invention provides a novel mixtureof, deuterium enriched compound of formula I or a pharmaceuticallyacceptable salt thereof, wherein the abundance of deuterium in R₁-R₃,R₉, and R₁₂-R₁₃ is at least 17%. The abundance can also be (a) at least33%, (b) at least 50%, (c) at least 67%, (d) at least 83%, and (e) 100%.

In another embodiment, the present invention provides a novel mixtureof, deuterium enriched compound of formula I or a pharmaceuticallyacceptable salt thereof, wherein the abundance of deuterium in R₄-R₆ isat least 33%. The abundance can also be (a) at least 67%, and (b) 100%.

In another embodiment, the present invention provides a novel mixtureof, deuterium enriched compound of formula I or a pharmaceuticallyacceptable salt thereof, wherein the abundance of deuterium in R₇-R₈ isat least 50%. The abundance can also be (a) 100%.

In another embodiment, the present invention provides a novel mixtureof, deuterium enriched compound of formula I or a pharmaceuticallyacceptable salt thereof, wherein the abundance of deuterium in R₁₀-R₁₃is at least 25%. The abundance can also be (a) at least 50%, (b) atleast 75%, and (c) 100%.

In another embodiment, the present invention provides novelpharmaceutical compositions, comprising: a pharmaceutically acceptablecarrier and a therapeutically effective amount of a deuterium-enrichedcompound of the present invention.

In another embodiment, the present invention provides a novel method fortreating multiple myeloma comprising: administering to a patient in needthereof a therapeutically effective amount of a deuterium-enrichedcompound of the present invention.

In another embodiment, the present invention provides an amount of adeuterium-enriched compound of the present invention as described abovefor use in therapy.

In another embodiment, the present invention provides the use of anamount of a deuterium-enriched compound of the present invention for themanufacture of a medicament (e.g., for the treatment of multiplemyeloma).

The present invention may be embodied in other specific forms withoutdeparting from the spirit or essential attributes thereof. Thisinvention encompasses all combinations of preferred aspects of theinvention noted herein. It is understood that any and all embodiments ofthe present invention may be taken in conjunction with any otherembodiment or embodiments to describe additional more preferredembodiments. It is also to be understood that each individual element ofthe preferred embodiments is intended to be taken individually as itsown independent preferred embodiment. Furthermore, any element of anembodiment is meant to be combined with any and all other elements fromany embodiment to describe an additional embodiment.

Definitions

The examples provided in the definitions present in this application arenon-inclusive unless otherwise stated. They include but are not limitedto the recited examples.

The compounds of the present invention may have asymmetric centers.Compounds of the present invention containing an asymmetricallysubstituted atom may be isolated in optically active or racemic forms.It is well known in the art how to prepare optically active forms, suchas by resolution of racemic forms or by synthesis from optically activestarting materials. All processes used to prepare compounds of thepresent invention and intermediates made therein are considered to bepart of the present invention. All tautomers of shown or describedcompounds are also considered to be part of the present invention.

“Host” preferably refers to a human. It also includes other mammalsincluding the equine, porcine, bovine, feline, and canine families.

“Treating” or “treatment” covers the treatment of a disease-state in amammal, and includes: (a) preventing the disease-state from occurring ina mammal, in particular, when such mammal is predisposed to thedisease-state but has not yet been diagnosed as having it; (b)inhibiting the disease-state, e.g., arresting it development; and/or (c)relieving the disease-state, e.g., causing regression of the diseasestate until a desired endpoint is reached. Treating also includes theamelioration of a symptom of a disease (e.g., lessen the pain ordiscomfort), wherein such amelioration may or may not be directlyaffecting the disease (e.g., cause, transmission, expression, etc.).

“Therapeutically effective amount” includes an amount of a compound ofthe present invention that is effective when administered alone or incombination to treat the desired condition or disorder. “Therapeuticallyeffective amount” includes an amount of the combination of compoundsclaimed that is effective to treat the desired condition or disorder.The combination of compounds is preferably a synergistic combination.Synergy, as described, for example, by Chou and Talalay, Adv. EnzymeRegul. 1984, 22:27-55, occurs when the effect of the compounds whenadministered in combination is greater than the additive effect of thecompounds when administered alone as a single agent. In general, asynergistic effect is most clearly demonstrated at sub-optimalconcentrations of the compounds. Synergy can be in terms of lowercytotoxicity, increased antiviral effect, or some other beneficialeffect of the combination compared with the individual components.

“Pharmaceutically acceptable salts” refer to derivatives of thedisclosed compounds wherein the parent compound is modified by makingacid or base salts thereof. Examples of pharmaceutically acceptablesalts include, but are not limited to, mineral or organic acid salts ofthe basic residues. The pharmaceutically acceptable salts include theconventional quaternary ammonium salts of the parent compound formed,for example, from non-toxic inorganic or organic acids. For example,such conventional non-toxic salts include, but are not limited to, thosederived from inorganic and organic acids selected from 1,2-ethanedisulfonic, 2-acetoxybenzoic, 2-hydroxyethanesulfonic, acetic,ascorbic, benzenesulfonic, benzoic, bicarbonic, carbonic, citric,edetic, ethane disulfonic, ethane sulfonic, fumaric, glucoheptonic,gluconic, glutamic, glycolic, glycollyarsanilic, hexylresorcinic,hydrabamic, hydrobromic, hydrochloric, hydroiodide, hydroxymaleic,hydroxynaphthoic, isethionic, lactic, lactobionic, lauryl sulfonic,maleic, malic, mandelic, methanesulfonic, napsylic, nitric, oxalic,pamoic, pantothenic, phenylacetic, phosphoric, polygalacturonic,propionic, salicyclic, stearic, subacetic, succinic, sulfamic,sulfanilic, sulfuric, tannic, tartaric, and toluenesulfonic.

EXAMPLES

Table 1 provides compounds that are representative examples of thepresent invention. When one of R₁-R₁₃ is present, it is selected from Hor D.

1

2

3

4

5

6

7

Table 2 provides compounds that are representative examples of thepresent invention. Where H is shown, it represents naturally abundanthydrogen.

8

9

10

11

12

13

14

Numerous modifications and variations of the present invention arepossible in light of the above teachings. It is therefore to beunderstood that within the scope of the appended claims, the inventionmay be practiced otherwise that as specifically described herein.

What is claimed is:
 1. A deuterium-enriched compound of formula I or apharmaceutically acceptable salt thereof:

wherein R₁-R₁₃ are independently selected from H and D; and theabundance of deuterium in R₁-R₁₃ is at least 8%.
 2. A deuterium-enrichedcompound of claim 1, wherein the abundance of deuterium in R₁-R₁₃ isselected from at least 8%, at least 15%, at least 23%, at least 31%, atleast 38%, at least 46%, at least 54%, at least 62%, at least 69%, atleast 77%, at least 85%, at least 92%, and 100%.
 3. A deuterium-enrichedcompound of claim 1, wherein the abundance of deuterium in R₁-R₃ isselected from at least 33%, at least 67%, and 100%.
 4. Adeuterium-enriched compound of claim 1, wherein the abundance ofdeuterium in R₉ and R₁₂-R₁₃ is selected from at least 33%, at least 67%,and 100%.
 5. A deuterium-enriched compound of claim 1, wherein theabundance of deuterium in R₁, R₉, and R₁₂-R₁₃ is selected from at least17%, at least 33%, at least 50%, at least 67%, at least 83%, and 100%.6. A deuterium-enriched compound of claim 1, wherein the abundance ofdeuterium in R₄-R₆ is selected from at least 33%, at least 67%, and100%.
 7. A deuterium-enriched compound of claim 1, wherein the abundanceof deuterium in R₇-R₈ is selected from at least 50% and 100%.
 8. Adeuterium-enriched compound of claim 1, wherein the abundance ofdeuterium in R₁₀-R₁₃ is selected from at least 25%, at least 50%, atleast 75%, and 100%.
 9. A deuterium-enriched compound of claim 1,wherein the compound is selected from:


10. A deuterium-enriched compound of claim 1, wherein the compound isselected from:


11. An isolated deuterium-enriched compound of formula I or apharmaceutically acceptable salt thereof:

wherein R₁-R₁₃ are independently selected from H and D; and theabundance of deuterium in R₁-R₁₃ is at least 8%.
 12. An isolateddeuterium-enriched compound of claim 11, wherein the abundance ofdeuterium in R₁-R₁₃ is selected from at least 8%, at least 15%, at least23%, at least 31%, at least 38%, at least 46%, at least 54%, at least62%, at least 69%, at least 77%, at least 85%, at least 92%, and 100%.13. An isolated deuterium-enriched compound of claim 11, wherein theabundance of deuterium in R₁-R₃ is selected from at least 33%, at least67%, and 100%.
 14. An isolated deuterium-enriched compound of claim 11,wherein the compound is selected from:


15. An isolated deuterium-enriched compound of claim 11, wherein thecompound is selected from:


16. A mixture of deuterium-enriched compounds of formula I or apharmaceutically acceptable salt thereof:

wherein R₁-R₁₃ are independently selected from H and D; and theabundance of deuterium in R₁-R₁₃ is at least 8%.
 17. A mixture ofdeuterium-enriched compounds of claim 16, wherein the compounds areselected from:


18. A mixture of deuterium-enriched compound of claim 16, wherein thecompounds are selected from:


19. A pharmaceutical composition, comprising: a pharmaceuticallyacceptable carrier and a therapeutically effective amount of a compoundof claim 1 or a pharmaceutically acceptable salt form thereof.